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Bligh & Dyer
method of total lipid extraction |
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Bligh, E. G. &
Dyer, W. J. (1959) A rapid method of total lipid extraction and
purification. Can. J. Biochem. Physiol. 37: 911-917. |
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Sample at 0-4°C. |
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Add 3.75 volumes
of chloroform/methanol (1:2). |
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Vortex 90 s. Centrifuge. |
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Add 1.25 volumes of chloroform and
1.25 volumes of water. |
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Vortex 60 s. Centrifuge. |
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Extract aqueous
phase (upper) with 2 volumes of chloroform. |
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Mix organic phases.
Dry with anhidrous sodium sulfate (4°C, 16 h). |
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Folch
method of total lipid extraction |
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Folch, J., Lees, M., & Stanley,
G. H. S. (1957) A simple method for the isolation and purification
of total lipides from animal tissues. J. Biol. Chem. 226: 497-509. |
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Tissue homogenate at 0-4°C. |
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Add 19 volumes of chloroform/methanol
(2:1). Filter. |
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Wash lipid extract with 0.2 volumes
of water. Centrifuge. Discard aqueous phase. |
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Wash organic phase twice with 0.2
volumes of chloroform/methanol/water (3:48:47). |
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Dry with anhidrous sulfate (4°C,
16 h). |
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Isolation
of lysophospholipids |
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Aqueous sample at 0-4°C. |
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Add 1 volume of water/hydrochloric
acid (19:1) and 1 volume of n-butanol. |
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Vortex and centrifuge. Keep the
organic phase (upper). |
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Extract the lower aqueous phase
with 0.5 volumes of n-butanol. |
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Mix the organic phases (n-butanol
evaporates very slowly!). |
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