Lipid Chemistry    Thin-layer chromatography of lipids - Solvent systems currently in use.  Bligh & Dyer method of total lipid extraction - The classic among the classics.  Folch method of total lipid extraction - Indicated when the starting material is a tissue homogenate.  Isolation of lysophospholipids - Extraction with n-butanol.  Determination of phospholipid phosphorus - Simplified method for phosphorus quantities < 50 nmol.  Belfrage method of fatty acid extraction - To extract fatty acids from mixtures with neutral glycerides.  Dole assay - To separate fatty acids from mixtures with phospholipids.  Separation of phospholipid subclasses - Converting phospholipids into diradyl benzoates.      Cellular Biochemistry    Measurement of arachidonic acid release from cells - Utilizing radiolabeled fatty acid.      Enzyme Assays    Phospholipid fatty acid incorporation - Arachidonoyl-CoA synthetase and CoA-dependent transferase.  Phospholipase A2 - Assays currently in use.  Phosphatidate phosphohydrolase - Useful for both Mg2+-dependent and -independent activities.